Macrolide antibiotics (Macrolide antibiotics, MALs) are a class of antibiotics produced by Actinomycetes or Micromonospora. MALs have become one of the fastest growing antibiotics in the world in terms of demand and sales. Because MALs have a broad-spectrum antibacterial effect and can resist Gram-positive bacteria, Mycoplasma and some Gram-negative bacteria, they are widely used in the treatment of respiratory and infectious diseases of pigs, cattle, sheep, shrimp and poultry, or It can be used as a feed additive at low doses to promote animal growth and development. The residues of macrolide antibiotics in food are likely to cause the proliferation of allergic strains carrying drug-resistant factors. Like other veterinary drugs, the monitoring and control of macrolides in animal-derived foods has been highly valued by many countries, including the Chinese government. Ministry of Agriculture Announcement No. 235 stipulates that the maximum residue limit (MRL) of erythromycin in animal tissues, milk and eggs is 40-200 μg / kg; the MRL of tilmicosin in animal tissues and milk is 50-500 μg / kg; the MRL of Qinolin in animal tissues, milk and eggs is 50-200 μg / kg.
This solution establishes a method for the rapid determination of macrolide antibiotics in pork using Shimadzu LC-30A and triple quadrupole mass spectrometer LCMS-8040. Eight macrolide antibiotics were quickly separated and detected within 4 minutes. Spiramycin, tilmicosin at 5-200 μg / L; oleandromycin, triclosin, berimycin, erythromycin, cross-samycin, roxithromycin at 1-500 μg / L The linearity in the concentration range is good, and the correlation coefficients of the standard curves are above 0.9996; precision experiments are performed on the mixed standard solutions of 5 μg / L, 20 μg / L, and 200 μg / L, and the retention time and peak area of ​​6 consecutive injections are relative The standard deviations are below 1.87% and 5.04% respectively, and the system precision is good.
For more details, please click "Ultra High Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry for Detection of Macrolide Antibiotics in Pork"
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Shimadzu Enterprise Management (China) Co., Ltd. is a local legal person company established in China in 1999 by Shimadzu Corporation to expand the scale of its business in China.
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