BIM Commentary: Recovery of Enzyme-Linked Immunoassay Kit

The recovery rate is the degree of loss of the analyte in the sample analysis process. The less the loss, the higher the recovery rate. If the standard solution is 1 PPM, it is 1 mg/L, and the scale data is 0.99 mg/L. Your recovery rate is 99%. This has a close relationship with the real ingredients, indicating the correctness of the method. For example, the total inorganic chlorine content in the water is determined. The sample water contains 20 mg/L of inorganic chlorine, 100 mL of the sample water sample is taken, and 0.1 mL of the inorganic chlorine-containing standard sample with a concentration of 10 mg/mL is added, and the volume change is ignored in the measurement. When the inorganic chlorine was 2.98 mg/L, the recovery was considered to be 98%.

If the recovery is close to 100%, then the accuracy of this kit is high. Simply put, it is the ratio of added and checked out. For example, you know that A is in the sample, but the result you detected by ELISA is B, and B/A is the recovery rate. The relative recovery rate may be higher than 100%. If it is just saying "loss level", it is really incorrect. The recovery rate is not necessarily 100% or less.

For quantitative testing, it is mainly a verification of accuracy. The relative recovery rate may be higher than 100%. If it is just saying "loss level", it is actually not accurate. It should be said that the closer the recovery rate is to 100%, the higher the accuracy of this kit.

BIM Commentary: Recovery of Enzyme-Linked Immunoassay Kit

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