Fluorescence microscopy technology first finds live cell "internal clock"

According to a report by the physicist organization network on the 11th, American scientists used advanced fluorescence microscopy technology to conduct a dynamic study on the shape changes of human nucleus in living cells for the first time, and found that the nucleus showed rapid volatility. This "internal clock signal" sign For the first time, humans have found physical properties that characterize cell cycle changes, providing new ways to understand the composition of life and the causes of disease.

Previous studies have only extracted cells that have developed to a certain point in the life cycle, and the extracted cells are already in a state of cessation of division or "death". Through such research, scientists have known that in a cell life cycle. The shape and size of the nucleus will change dramatically. However, due to technical limitations, it is impossible to track changes in the nucleus of living cells, so whether the shape of the nucleus changes during various periods of the cell life cycle has not been known.

The latest study, published in the Proceedings of the National Academy of Sciences, said that Alexandra Zdovica, an associate professor of physics at New York University, and colleagues used fluorescence microscopy to observe the shape of the nucleus in living cells at various points in time. Subtle changes. They found a type of activity that was not detected before in the human nucleus: under a fluorescence microscope, the nuclear envelope flickers every few seconds, meaning that its size fluctuates every few seconds, and more importantly, The magnitude of this shape fluctuation shows a decreasing trend throughout the cell cycle.

Zdovica explained that the nuclear envelope is the outermost structure of the nucleus, which protects the DNA molecules in the nucleus from damage and is a channel for material exchange. If the nuclear envelope is inaccurate in structure and function, it can lead to developmental genetic diseases such as cardiomyopathy, muscular dystrophy and cancer. He said: "The process of nuclear envelope fluctuation can be regarded as the internal clock of the cell. Its fluctuation signal can tell people which stage of the life cycle. The mechanism behind it is important for understanding the important components of healthy cells and the etiology of diseased cells.

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