Objective: To standardize the operation method of SW-CJ-1F medical purification workbench. Ensure aseptic operation and maintain the quality of the test.
Scope of application: Suitable for aseptic operation of SW-CJ-1F medical purification workbench. Responsibilities: The hygiene inspection room is responsible for implementation. content:
I. Overview: SW-CJ-1F medical purification workbench is one of the most important equipments used for aseptic operation such as hygiene inspection and microbial pure culture.
Second, the operating procedures:
1. Before use, it should be carefully checked, debug various performances and conditions of the cleaning workbench, carefully check and debug the power supply, voltage is intact and stable, and use 75% alcohol to carefully clean the cleaning work before use.
2. Put the instrument supplies needed for the experiment into the workbench, turn on the main power supply, turn off the ventilation table and the general lighting switch, turn on the UV lamp for 30 minutes, and perform ultraviolet sterilization and disinfection. 
3. Turn off the UV lamp, turn on the illumination, switch the fan, adjust the wind to the appropriate size, wait for more than 30min to drain the ozone.
4. Wear a gown, wear a mask, and a hat. Hands and wrists are disinfected with 75% alcohol. 5. Ignite the alcohol lamp. In the clean bench, avoid placing too many items. The straws, droppers, test tubes, and culture bottles used are pre-sterilized.
6. Open the various types of caps before overheating to fix the dust; open the mouth of the bottle and the mouth of the test tube to pass the flame. The tweezers should be burned by flame before use.
7. The clean bench of the horizontal fan should be inclined, and the bottle opening should be avoided as far as possible. 8. The same pipette or dropper should not be used continuously for several different cell lines; the pipette of the suction medium should be 0.5cm away from the culture bottle or the test tube to avoid sticking into the mouth of the culture bottle or the test tube; to prevent the cell line Mixed pollution.
9. The liquid leaking on the culture bottle or on the table is immediately wiped with an alcohol cotton ball.
10. After the experiment is completed, clean the work and keep the clean bench top clean and free of residue. Turn off the power after turning off the ventilation and lighting equipment. Take away all the rubbish when leaving the room.
Third, cleaning and maintenance procedures
1. After performing the aseptic experiment in the clean bench, the generated garbage should be treated separately according to the type. The discarded culture should be placed in a sterilization pot at 121 ° C for 15 minutes, and the experimental products should be soaked in 5%. After 24 hours in the aqueous solution of Su, the rinse was taken out.
2. The net air filter is replaced once a year. If the blockage is serious, the replacement cycle can be shortened appropriately.
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